Rhamnolipid production from waste cooking oil using newly isolated halotolerant Pseudomonas aeruginosa M4

This study isolated a novel halotolerant Pseudomonas aeruginosa M4, that was able to degrade oil and produce rhamnolipids. Various carbon sources, nitrogen inoculum ratio, pH, temperature were tested optimize the degradation conditions. The highest rate of 85.20% lipase activity 23.86 U/mL obtained under optimal conditions (5% at 35 °C pH 8). components products different times analyzed explore mechanism by GC-MS. Short chain fatty acids acetic n-butyric primary intermediates. maximum rhamnolipid concentration 1119.87 mg/L produced when P. M4 used waste cooking (WCO) as sole source. Rhamnose precursors synthesized from glycerol, hydrolysis product WCO. R-3-hydroxyalkanoate de novo using acetyl-CoA ?-oxidation acids. In addition, had good salt tolerance up 70 g/L. findings show is valuable biosurfactant producer in treatment